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A new simple medium for in vitro sperm activation of asthenozoospermic patients using direct swim-up technique | ||
| KUFA MEDICAL JOURNAL | ||
| Article 1, Volume 14, Issue 1, 2011, Pages 67-75 | ||
| Authors | ||
| Assist. Prof. Dr. Muhammad-Baqir M-R. Fakhrildin; and Nisreen Khazal Flayyih | ||
| Abstract | ||
| This study was designed to compare the results of sperm parameters for asthenozoospermic patients pre- and post-activation in vitro using a new simple medium and direct swim-up technique. Fifty asthenozoospermic patients were involved in the present study with age within range 22-41 years and duration of infertility 2-10 years. From each patient, semen sample was taken and seminal fluid analysis (SFA) was done. A new simple medium was prepared based on Ringer solution supplemented with additives sodium pyruvate and human serum albumin (5% or 10%) which referred as SMART medium (Simple Medium for Assisted Reproductive Techniques; SMART). SMART and Earl's media were used for in vitro sperm activation by direct swim-up technique. Sperm concentration, sperm motility (%), sperm grade activity (%), normal sperm morphology (%) and sperm agglutination (%) were assessed. Crude data were statistically analyzed. From the results of present study, it was appeared that most sperm parameters were significantly (P<0.05) enhanced post-activation in vitro as compared to pre-activation using SMART medium by direct swim-up technique. After in vitro sperm activation, the percentages of progressive sperm activity with grade A, total progressive sperm motility (grades A and B) and normal sperm morphology were significantly increased (P<0.05) in both treated groups as compared to control groups. However, highly significant (P<0.01) and positive correlations for sperm concentration and normal sperm morphology (%) were observed among groups of pre- and post-activation. It was concluded that the SMART medium was suitable for enhancement of sperm parameters of asthenozoospermic patients using direct swim-up technique. Further studies are needed to evaluate effects of SMART medium on successful rate of intrauterine insemination. | ||
| Keywords | ||
| Asthenozoospermia; sperm; activation; SMART medium | ||
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