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THE RELATIONSHIP BETWEEN THE DILUENT PROVISION OF GREEN TEA INFUSION AND LIPID PEROXIDATION DURING IN VITRO STORAGE OF ROOSTERS' SEMEN | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 38, Issue 2, 2007, Pages 141-147 | ||
| Authors | ||
| I.A.Abdul- Hassan; R. H. Razuki; H. J. Al - Daraji | ||
| Abstract | ||
| An experiment was conducted to determine the effect of supplementation roosters' semen diluent with green tea infusion on semen quality during in vitro storage for up to 72 h. Sixty males (White Layers, 24 wk of age) divided in six groups of 10 cocks were used for experimentation. Semen samples were collected twice a week from all roosters during the whole period of experiment (12 weeks). Treatment groups were as follows: Tl : fresh semen (control group). 12 : semen diluted 1:2 with Lake diluent (LD) alone, and T3 - T6 : semen samples diluted 1:2 with LD supplemented with 2, 4, 6 or 8 ml of green tea infusion / 100 ml of diluent, respectively. Results indicated that the inclusion of green tea infusion in LD diluent resulted in a significant decrease (p < 0.05) in percentages of dead and abnormal spermatozoa and acrosomal abnormalities and significant increase (p < 0.05) in mass activity and individual motility in comparison with Tl and T2 groups when semen samples were evaluated directly after collection (Oh) or after 24, 48 or 72 h of in Vitro storage at 4 - 6 °C. There were no significant differences (p > 0.05) between T3, T4 and T6 groups concerning all semen characteristics involved in this study. However, T5 group (6 ml green tea / 100 ml of diluent) recorded the lowest values of percentages of dead and abnormal spermatozoa and acrosomal abnormalities and highest values of mass activity and individual motility as compared with other treatments of green tea (T3, T4 and T6). In conclusion, the addition of green tea infusion into semen diluent can be used successfully for suppress the detrimental effects of lipid peroxidation that naturally occurred during in xitro storage of avian semen. | ||
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