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Sero-Diagnosis of Toxoplasmosis among pregnant women and immunocompromised patients | ||
| KUFA MEDICAL JOURNAL | ||
| Article 1, Volume 13, Issue 2, 2010, Pages 268-275 | ||
| Author | ||
| Prof. Dr. Baqur A. Sultan | ||
| Abstract | ||
| Summary The study is conducted to detect the prevalence of anti-Toxoplasma gondii antibodies among pregnant women as well as immuncocompromised patients like diabetic patients, leukemic, patients with malignancy and chronic renal failure, with Latex agglutination test (LAT), IgG & IgM Enzyme immunoassay, and IgG & IgM Dipstick dye immunoassay. The total number of positive cases of toxoplasmosis was 210 (52.2%), 79 (19.6%), 193 (48%), 87(31.9%) and 163(59.9%) by LAT, IgM-EIA, IgG-EIA, IgM-DDIA and IgG-DDIA respectively. Introduction: Toxoplasma gondii is globally distributed pathogen for human and most of the infected people being clinically asymptomatic, but it causes significant morbidity and mortality in developing fetuses and in immunocompromised patients(1,2) . Toxoplasma gondii is one of the major opportunistic infection agent in immunocompromised cases such as haemodialytic, leukemic, Hodgkin's and non-Hodgkin's disease and diabetic patients, (3). The effect of Toxoplasma infection may differ depending on such factors as individual genetic predisposition, the state of the immune system, the dose, and the virulent of the infecting strain (4). Clinical signs of toxoplasmosis are non specific and not sufficiently characteristic, so diagnosis of Toxoplasma gondii infection in human is made by biological, serological, histological and molecular methods (5). Latex Agglutination Test (LAT) is a rapid agglutination procedure for the qualitative and semiquantative determination of anti-Toxoplasma antibodies and it is made by specific agglutination of latex suspension for T. gondii antigens coated polystyrene with Toxoplasma antibodies(6). Enzyme Immune Assay (EIA) could be used in the detection of the isotype IgA, IgM, IgG (7). The fast dipstick dye Immuno assay (DDIA) is simple, cheap, sensitive and specific for the detection of anti- Toxoplasma IgG or IgM antibodies and generally agree closely with the results of EIA(8). The aim of the study is to investigate the prevalence of anti-Toxoplasma gondii antibodies among pregnant women as well as immunocompromised patients by LAT, IgG-EIA, IgM-EIA, IgG-DDIA and IgM-DDIA. | ||
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