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Partially Purification and Biological Properties of Shigella dysenteriae Type 1 Shiga Toxin | ||
| KUFA MEDICAL JOURNAL | ||
| Article 1, Volume 11, Issue 1, 2008, Pages 121-131 | ||
| Author | ||
| Ali M. Al-Muhana | ||
| Abstract | ||
| A toxin from Shigella dysenteriae type 1 (strain BB46) was partially purified. The steps used to purified the toxin included intermittent sonic oscillation of bacterial grown in modified synicase broth, ammonium sulphate precipitation, and chromatography on diethylaminoethyl sephadex and carboxymethyl sephadex. The partial purified preparation had a specific activity of 41.8×105 CD50/mg protein in Vero cells. The clinicopathological effects of intravenous administered partially purified toxin in rabbits and intraperitonial injection in mice were studied. The LD50 values were 0.2 and 40 µg/kg body weight in rabbits and mice, respectively. The clinical feature included diarrhea in some animals and a progressive flaccid paralysis, usually culminating in death. The histopathology of rabbits was characterized by edema and hemorrhage in the spinal cord and edema in the brain. | ||
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