Determination Of Human Cytomegalovirus Proteins In The Paraffin-Embedded Tissues Of Colorectal Adenocarcinoma.

AL-Mosawi Ph.D, Afer

	Determination Of Human Cytomegalovirus Proteins In The Paraffin-Embedded Tissues Of Colorectal Adenocarcinoma.
KUFA MEDICAL JOURNAL
Article 1, Volume 11, Issue 1, 2008, Pages 334-342
Author
afer AL-Mosawi Ph.D
Abstract
Some epidemiological studies have suggested a possible link between human cytomegalovirus infection and various malignancies particularly colorectal adenocarcinoma. The current study investigates whether HCMV participates in human colorectal tumorigenesis by the detection of HCMV proteins within epithelial cells of colorectal carcinoma using Immunohistochemistry (IHC). We obtained formalin – fixed, paraffin – embedded specimens of adenocarcinoma, villous adenoma, and normal tissues from the margins of the excision as a control. A total number of 60 specimens were classified into three groups, malignant group, 25 male &10 female, benign group, 10 male & 5 female and control group, 10 specimens. These specimens were tested by IHC to detect the presence of HCMV proteins, early protein (Biogenex, USA) and a mixture of monoclonal antibodies of early and immediate early proteins (Dakocytomation, Denmark). The results of IHC assay showed specific nuclear and cytoplasmic reaction of HCMV proteins within the epithelial cells of colorectal adenocarcinoma (88.5%),and villous adenoma (53.3%), in addition to that no nuclear or cytoplasmic reaction were showed in any case of group C (control).Our data justify further studies to establish whether HCMV interfere with the pathogenesis of colorectal adenocarcinoma.

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