Behavior of Creatine Kinase Isoenzymes  in Hepatic Diseases

J.  Al, Fadhil; Toma,

	Behavior of Creatine Kinase Isoenzymes in Hepatic Diseases
KUFA MEDICAL JOURNAL
Article 1, Volume 11, Issue 1, 2008, Pages 501-507
Authors
Fadhil J. Al;
Abstract
Serum creatine kinase isoenzymes were determined in 107 patients with various hepatic disorders (29 with hepatocellular carcinoma; 26 with alcoholic cirrhosis; 33 with primary biliary cirrhosis and 19 with hepatic failure) and compared with that found in 34 control individuals with the same age which ranged between 25–76 years. Elevations in total serum CK activity levels were observed in 69%; 76.9%; 51.5% and 63.2% of the cases studied and the maximum value was reached to 10.9 I.U./ml; 9.4 I.U./ml; 8.2 I.U./ml and 13.4 I.U./ml respectively as compared with that found in control group (4.1 ± 1.2 I.U./ml). Serum CK isoenzymes have been separated from these types of hepatic patients by mini-column ion-exchange chromatography. CK-MM isoenzyme was found in sera of all cases studied in a variable degrees, whereas CK-BB isoenzyme was separated from 79.3% of hepatocellular carcinoma; 73.1% of alcoholic cirrhosis; 60.1% of primary biliary cirrhosis and 79% of hepatic failure and its activity levels was reached to 25%; 19%; 15% and 24% of total serum CK activity receptively. CK-MB isoenzyme was also observed and separated only from sera of hepatic disorders when massive myocardium damage is occurring. Therefore the detection and measurement of CK-BB isoenzyme may act as a good biochemical marker and to give some information about the severity of liver diseases and also to differentiate between them. Column isoelectric focusing was also performed and the isoelectric points of each of CK isoenzyme separated from sera of different types of hepatic disorders have been determined.

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