A Study of Physiological and Biochemical Effects of Eruca Stavia Leaves extract on the Blood of Albinino Male Rats (Rattus Norvegicus) Exposed to Oxidative Stress.

J. Abdalrahman, Sahib; M. Mubark, Zaid; M. Rahem, Salih

	A Study of Physiological and Biochemical Effects of Eruca Stavia Leaves extract on the Blood of Albinino Male Rats (Rattus Norvegicus) Exposed to Oxidative Stress.
Al-Ma'mon College Journal
Article 1, Volume 0, Issue 16, 2018, Pages 240-254
Authors
Sahib J. abdalrahman; Zaid M. mubark; Salih M. Rahem
Abstract
The aim of the present study was to invegstigate some physiological and biochemical effects of the aqueous extract of Eruca Stavia leaves on blood of Albino rats (Rttus norveqicus) exposed to oxidative stress induced by hydrogen peroxide (0.5%) with drinking water for (21) days Rats (245-285g in weight and 12-14 week k age) were randomly divided to (4) groups (treatments) each with five replicates. These groups are: 1-Control group (rats without any treatment). 2-H2O2 group (rats treatment with H2O2). 3-Eruca Stavia leaves extract group (rats treated with Eruca Stavia leaf extract 250mg/kg b.w) 4-Eruca Stavia leaf extract + H2O2 group . Sub cutaneous injection (singl dose every day for (21) day method used in this study. The results showed compared with the control the oxidative stress induced by H2O2 caused significant increase (p<0.05) in total count of leukocytes, differential count of leukocytes and the level of blood sugar (glucose), total cholesterol, triglycerides (TG), low density lipoprotein (LDL-C), very low density lipoprotein, atherogenic index (first and second) urea and Malondialdehyde (MDA). This stress showed as well, a significant decrease (p<0.05) in Hb. PCV, hig density lipoprotein (HDL-C), total protein , Albumin and glutathione. The treatment of Eruca Stavia leaf extract (group 4) and compared with the treatment of H2O2 (group 2) showed significant decrease in lymphocytes number and in the level of blood sugar (glucose), total cholesterol T.G, LDL-C, VLDL-C and atherogenic index (first and second), blood urea, and MDA. Also this treatment showed asignificant increase of the eosinophils, mancytes, basophils, and HDL-C. However this treatmeant showed non signiticant difference in the levels of Hb, PCV. And Total count of feukocytes and of bumin. Rats treatment with Eruca Stavia leaf extract alone (not treated before with H2O2 ) showed no clear difference from the control group though some improvement was observed in some physiological and biochemical characterts of bood. The above results revealed that the Eruca Stavia leaf extract used in this study play an important role in decreasing the harmful effect of the free radicals in the animals studied.
Keywords
Eraca sativa; Rattus norvegicus

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