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Evaluation of Dot. ELISA test for Diagnosis Visceral Leishmaniasis in Infected Children | ||
| Ibn Al-Haitham Journal For Pure And Applied Science | ||
| Article 1, Volume 22, Issue 4, 2009, Pages 10-15 | ||
| Authors | ||
| A. A. J. J. Al- Saadi; R. S. Al-Sady; W. R. Ali | ||
| Abstract | ||
| For the first time in Iraq, this study was conducted to evaluate the usefulness of Dot.ELISA, for detecting anti - Leishmania donovani antibodies in serum samples from suspected patient (children under 8 years ) with Visceral Leishmaniasis V.L.. Sera from 73 V.L. , 60 Healthy controls, and 57 patient with other parasitic diseases other than V.L. (Amoebiasis, Giardiasis , Toxoplasmosis, Schistosomiasis , Hydatidosis, Ascariasis , Lupus Erythromatosus , Viral Hepatitis, and Cutaneous Leishmaniasis) were examined. Anti Leishmania donovani antibodies detected in 71 out of 73 suspected Visceral Leishmaniasis . Data of this study showed that infection in male group was more than female group. Result of this study revealed a high prevalence of patients with V.L. infection at the age range 1 - 2.9 and 3 - 4.9 years when compared with other range groups (P>0.01). A cross – reaction was found with sera Lupus Erythromatosus, No cross - reaction was found with sera from other disease . Sensitivity was determined as 97.3 %, and Specificity was found to be 95.7% . Positive and Negative the Values Predictive of test were 96% and 97.4% Dot .ELISA is simpler, Serodiagnosis Rapid, sensitive and specific, Its use in respectively. Seeing that of V.L. can be recommended | ||
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