DETECTION OF GIBBERELLINS AND AUXINS LIKE COMPOUNDS AND THE PHYTOHORMONETHYLENE IN CULTURE FILTERATE OF THREE ISOLATES OF Trishoderma harzianum.

Abood, H.M.; Abood, M.R.; F.HSaeed,

	DETECTION OF GIBBERELLINS AND AUXINS LIKE COMPOUNDS AND THE PHYTOHORMONETHYLENE IN CULTURE FILTERATE OF THREE ISOLATES OF Trishoderma harzianum.
The Iraqi Journal of Agricultural Science
Article 1, Volume 39, Issue 2, 2008, Pages 12-18
Authors
H.M. Abood; M.R. Abood;
Abstract
This study was conducted to detect the efficiency of three local isolates of Trichoderma harzianum (T9, T26 and T28) in producing some plant growth enhancement hormones like gibberellins , auxins and ethylene. The results showed the ability of the three tested isolates T9, T26 and T28 in producing gibberellins and auxins when cultivated on potato sucrose broth medium for 14 days on 25±2 °C , but their production ability was varied significantly in producing the two studied hormones. The results of chemical analysis culture filtrates using (HPLC) High-Performance Liquid Chromatography technique showed the superior of isolate T9 in their ability to produce gibberellins compared to T26 and T28 which recorded 43.39 mg/L compared to 18.24 and 0.59 mg/L for isolates T26 and T28 respectively while isolate T26 recorded high efficiency in production auxins 22.3 mg/L compared to the productivity of isolate T9 and T28 which recorded 0.00 and 0.06 mg/L respectively.Gas chromatography technique showed the occurrence of ethylene in different quantities in me cultures of the three tested isolates of T. harzianum cultivated on Czapek's broth medium for four days on 25±2 °C . This results confirmed the hypothesis of phytohomones production which enhance plant growth by some isolates of Trichoderma spp to be usee! as an important tool for plant growth enhancement.

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