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EFFECT OF DIFFERENT TREATMENTS ON REGENERATION, GROWTH ANDACCLIMATIZATION OF PINEAPPLE PLANTLETS PRODUCED BY PLANT TISSUE CULTURE 1-MICROPROPAGATION VIA ADVENTITIOUS SHOOT REGENERATION FROM LEAVES SEGEMENTS CULTURE OF Ananas comosus .L.Merr.cv.Del Mont | ||
| Basrah Journal of Agricultural Sciences | ||
| Article 4, Volume 25, Issue 1, 2012, Pages 13-21 PDF (0 K) | ||
| DOI: 10.33762/bagrs.2012.54167 | ||
| Author | ||
| HUDA. A.Al-TAHA | ||
| Abstract | ||
| This study was conducted at laboratory of plant tissue culture - department of Horticulture and land scape design –College of Agriculture- Basra University, during the year of 2011. The aim was to find out the effect of two different levels of cytokins (BA) and oxins (NAA) on primary callus induction ,and then adventitious shoot initiation and formation of plantlet from leaf segments of pineapple plant produced through tissue culture protocol. Results revealed that the primary callus formation was increased with increasing of BA concentration in callus media. However, large quantity of primary callus was obtained in media supplemented with 7 and 10mg/l BA with constant level (0.5 mg /l) of NAA in solid media of MS .The subculture of calli was continued in the same media (2,3,5,7,and 10 mg / l) of BA three times during incubation in dark. Adventitious shoots were also regenerated from primary callus that was derived from leaf segments cultured in MS media with BA at concentration of 1 mg/l with NAA at concentration 0.1mg for two months in light. The Adventitious shoots regeneration was continued for 4, 6 and 8 months from beginning of primary callus culture in the same media for mass production of Ananas plant. Adventitious shoots were rooted in media contained half strength MS , 1 gm activated charcoal, 0,3 mg /l NAA and 0.1mg/l BA, for three months in light. Results also showed, that plantlets which were hardened in distilled water produced new white roots and then grown when they were planted in a medium of soil: Petmos 2; 1 and the survival rate was 100%, whereas, non-hardened plantlets had yellowish colored leaves with 30% surviving rate when they were cultured in the same media. | ||
| Keywords | ||
| Micropropagation; leaves culture; Ananas comosus; Merr | ||
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