Issa and A.H. Lafta, A., M. Sherif, I. (2006). Extraction and purification of gliadin from local variety wheat as specific antigen and using it in the enzyme linked immunosorbent assay (ELISA) as serological test diagnosis of celiac disease. , 24(1), 70-85.
A.H. Issa and A.H. Lafta; I.S. M. Sherif. "Extraction and purification of gliadin from local variety wheat as specific antigen and using it in the enzyme linked immunosorbent assay (ELISA) as serological test diagnosis of celiac disease". , 24, 1, 2006, 70-85.
Issa and A.H. Lafta, A., M. Sherif, I. (2006). 'Extraction and purification of gliadin from local variety wheat as specific antigen and using it in the enzyme linked immunosorbent assay (ELISA) as serological test diagnosis of celiac disease', , 24(1), pp. 70-85.
Issa and A.H. Lafta, A., M. Sherif, I. Extraction and purification of gliadin from local variety wheat as specific antigen and using it in the enzyme linked immunosorbent assay (ELISA) as serological test diagnosis of celiac disease. , 2006; 24(1): 70-85.

Extraction and purification of gliadin from local variety wheat as specific antigen and using it in the enzyme linked immunosorbent assay (ELISA) as serological test diagnosis of celiac disease

basrah journal of science
Article 1, Volume 24, Issue 1, 2006, Pages 70-85    PDF (0 K)
Authors
A.H. Issa and A.H. Lafta; I.S. M. Sherif
Abstract
Gliadin was extracted from local variety wheat (Ashuur) in which wheat grains were milled and sieved out and gluten was isolated by hand wash method then gliadin was extracted with alcohol, this crude gliadin was purified by gel filtration chromatography by sephadex G-100, then molecular weight and purity detected by Polyacrylamide gel electrophoresis and the results were four bands with molecular weights: 86245, 50118, 23307 and 12022 D.
Enzyme linked immunosorbent assay (ELISA) was used firstly to determine the optimum concentration of gliadin antigen with test serum by chequar-board, the chosen concentration was 2.5 µg/ml by using calibrators standards, controls and solutions of commercial kit (Pharmacia diagnostic kit).
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