CONJUGATIVE PLASMID-BORNE ANTIBIOTIC RESISTANCE GENES IN PSEUDOMONAS AEROGENOSA

Sulaiman Hamad, Ibrahim; Muhammed Ali, Sinaa; M. Hamzah, Haider

	CONJUGATIVE PLASMID-BORNE ANTIBIOTIC RESISTANCE GENES IN PSEUDOMONAS AEROGENOSA
basrah journal of science
Article 1, Volume 26, Issue 1, 2008, Pages 11-19 PDF (0 K)
Authors
Ibrahim Sulaiman Hamad; Sinaa Muhammed Ali; Haider M. Hamzah
Abstract
A total of 6 isolates of P. aeruginosa were isolated from premature unit, oxygen administration room and blood of patients admitted in pediatric hospital in Sulaimani. The isolates were identified as P. aeruginosa according to the diagnostic keys. All isolates were tested for susceptibility towards 10 different antibacterial drugs and they exhibited multiresistant for several choice antibiotics (amikacin, ampicillin, carbencillin, chloramphenicol, cefotaxime, neomycin, and trimethoprimsulfamethoxazole), with varied susceptible to rifampicin, gentamicin, and tetracycline. Agarose gel electrophoresis showed two different plasmid bands in P. aeruginosa isolate which is resistant to 7 antibiotics. One of these bands was ranged between 41 to 45 kbp and identified as conjugative plasmid and it confirmed through conjugation process with E. coli. Transconjugants were selected on agar plate containing antibiotics (rifampicin, cefotaxime, and ampicillin) and was recovered at frequencies in range of 10-5-10-6 per recipient. Data suggest that bacterial contamination of different units of pediatric hospital should be reduced or eliminated to minimize nosocomial infection risks related to transfer of antibiotic-resistant bacteria to other patients.
Keywords
Multiple; antibiotic; Resistant; aeruginosa; Conjugative plasmids

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