Androgenesis and Spontaneous Chromosome Doubling in Hordeum vulgare L.

Mirzaei, Maryam

	Androgenesis and Spontaneous Chromosome Doubling in Hordeum vulgare L.
Euphrates Journal of Agriculture Science
Article 1, Volume 3, Issue 0, 2011, Pages 248-252
Author
Maryam Mirzaei
Abstract
Abstract : Androgenic embryogenesis is a method to produce haploid plants. In this research androgenesis and spontaneous chromosome doubling of 2 barley genotypes (B1 and B2) were studied via isolated microspore culture. The experimental design was a completely randomized design with 3 replications. Donor plants were grown in desired conditions in Research Field of Razi University. The spikes were harvested in mid to late uninucleate stage and surface sterilized by 70% ethanol. The anthers were removed and placed in 0.3 M mannitol for 4 days and 25o C. After filtration, the microspores were collected via centrifugation. Microspores were cultured in FHG induced liquid medium with Phenylacetic acid and transferred to incubation conditions (dark and 25o C). Numbers of produced haploid embryos from 100 anthers were recorded after 3 weeks. The embryos subcultured to regeneration medium. Statistical analysis of embryogenesis and cytogenetic results showed that genotype significantly affected haploid embryogenesis and they were not significant in spontaneous chromosome doubling. Obtained results showed that B1 genotype had higher rank for haploid embryo formation (345.2) embryos from 100 anthers) and B2 was in lower percentage as produced 180.3 embryos from 100 anthers. The spontaneous chromosome doubling were 63 and 72%, respectively. Key words: Haploid embryogenesis, Spontaneous chromosome doubling, Genotype, Isolated microspore culture, Barley (Hordeum vulgare L.)

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