Effect of Crude Ellagic acid Extract from  Punica granatum pericarp on Mammary   Gland Carcinoma Transplanted in Female mice

M. Husaien, Shalal; M . Noori, Muhaned; Y.Kadhim, Aseel

	Effect of Crude Ellagic acid Extract from Punica granatum pericarp on Mammary Gland Carcinoma Transplanted in Female mice
Iraqi Journal of Cancer and Medical Genetics
Article 1, Volume 3, Issue 1, 2010, Pages 91-98
Authors
Shalal M. Husaien; Muhaned M . Noori; Aseel y.Kadhim
Abstract
This study was carried out to recognize the effect of the most important component in the pericarp of Punica granatum fruit which is known as crude Ellagic Acid. The toxic effect of Crude Ellagic Acid extract has been tested in vivo to determine the LD50 in normal mice, and the therapeutic effect in tumor transplanted mice. The qualitative chemical analysis showed that crude Ellagic Acid extracted contain Glycosides ,Tannin ,and Flavonoid while Steroid ,Terpen ,and Alkaloid were absent . This study showed intermediate toxicity of crude Ellagic Acid extract in normal laboratory mice in respect to LD50 which was 935.25 mg/kg .while the result of therapeutic experiments in tumor bearing mice showed reduction in tumor volume about 61.8% in those treated with the higher dose of Crude Ellagic Acid extract (93.525) mg/kg,and 97.4% in those treated with lower dose (46.762) mg/kg after 5 weeks from the onset of the treatment. Moreover histopathological study of the tumor mass at the end experiment indicated necrosis in the tumor mass of high dose –treated mice(93.525) mg/kg, but those treated with low dose(46.762)mg/kg of Crude Ellagic Acid showed infiltration of inflammatory cell in addition to necrosis in their tumor mass . All treated mice with high and low dose of crude Ellagic acid showed no histopathological effect in their internal organs including Liver, Spleen, and Kidney.
Keywords
Punica granatum pericarp

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