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Detection of Toxic Shock Syndrome Toxin Genes in Enterotoxin Producing Staphylococcus aureus Isolated from Food and Food's Workers | ||
| Journal of College of Education for Pure Science | ||
| Article 1, Volume 2, Issue 2, 2012, Pages 100-110 | ||
| Authors | ||
| Muhalab T; Raji T. Nasir; Yahya A. Abbas | ||
| Abstract | ||
| One hundred fifty isolates of Staphylococcus aureus were isolated from foods and food worker s, distributed as: 42 isolates from milk, 22 from salads, 17 from meat, 14 from local cheese, 12 from chicken , 30 from nasal swabs and 13 from body swabs of food workers. The ability of S. aureus isolates for enterotoxin production was detected by SET-RPLA Kit Oxoid, and showed that 80.6% of the isolates were positive for enterotoxin production. Susceptibility of the isolates to eleven antibiotics was detected. The isolates showed high resistance against methicillin, ampicillin and amoxicillin. The resistance ranged between 60 to 66.6%for cephalothin, ciprofloxacin, trimethoprim, cefotaxime, erythromycin and chloramphenicol. Resistance to vancomycin was 16%. Polymerase chain reaction technique was used for detection the presence of toxic shock syndrome toxin genes(TSST-genes) in the DNA of 100 isolates of S. aureus which were methicillin resistance and enterotoxin producers. They appeared that only six isolates were carried TSST-genes, four isolates from food workers and two from milk samples. | ||
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