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Anatomy study of adventitios buds of date palm (Phoenix dactylifera L.) c.v Barhi in vitro | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 36, Issue 1, 2010, Pages 31-37 PDF (0 K) | ||
| Author | ||
| Aqil.A.S.Al -Khalifa Usama N.J.Al-meer | ||
| Abstract | ||
| Vegetative micro propagation of date palm (Phoenix dactylifera L.) has been established through formation of adventitious buds from culture of white nodular callus in synthetic media containing high level of the cytokinin (2ip)(5 and 10 mg/l)for 6 months. Histological examination of the adventitious buds formation revealed the nodules in the subculture masses to be a pro buds initiated from meristematic cells located in the superficial layers of the organogenesis callus,it was found that buds initiated from compact aggregates cells proliferate through successive divisions into bipolar structures which appear as free nodules embedded in the callus mass. Upon transferring the buds formation tocultured on nutrient medium of (MS)salts full strength supplemented 30 g/l sucrose,0.25 g/l ctivated charcoal ,7 g/l agar,(1,2) mg/l NAA and cytokinins (2iP)(5,10)mg/l. completed their growth and developed into entire plantlets. | ||
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