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ISOLATED BACTERIA FROM CONTAMINATED CALLUS AND HEALTHY DATE PALM OFFSETS | ||
| Basrah Journal of Agricultural Sciences | ||
| Article 11, Volume 20, Issue 1, 2007, Pages 166-176 PDF (0 K) | ||
| DOI: 10.33762/bagrs.2007.56259 | ||
| Authors | ||
| ; Hadeel T.AL; Sabeh D.ALutbi; Zainab J.Madhi | ||
| Abstract | ||
| This study was conducted to determine types of bacteria contaminating three types of callus of date palm cultivars: Al – Barhee , Al- Khudrawi & Al- Hillawi ; in addition to shoot tips of offsets of date palm grown in Murashige – Skoog tissue culture medium . Six genera of bacteria were identified from contamination detected 24 –48 h. after growing cultures ( External contamination ) . These are : Bacillus (33.5%) , Staphylococcus (28.8%) , Agrobacterium (14.4%) , Pseudomonas (12.8%) , Serratia (6.7%) & Proteus (3.6%) . Bacillus was found axenically in 22% of cultures and Staphylococcus was also found axenically but in 15% of them ; these two genera were never isolated together from the same sample . Bacillus was the only genus isolated from contamination detected 4-8 weeks after growing cultures ( Internal contamination ) . Identification of the genus Bacillus showed the prevalence of the two species : B.subtilis & B. brevis from external contamination , and the species B . circulans from internal contamination . Intensity of internal bacteria has markedly increased in shoot tips of offsets of date palm with the increase of lawyer thickness adjacent to merrshimic shoot tips. | ||
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