EFFECT OF SOME CHEMICAL COMPOUNDS,  SURFACTANT MATERIALS AND COMMERCIAL DETERGENTS ON THE ACTIVITY OF LIPASE ENZYME PURIFIED FROM HEPATOPANCREASE OF CRAB     Portunus  pelagicus (L. 1758)

Y. J. Al-Sraji, Amar; M. H. AL-Shatty, Sabah; M. Al-Ali, Raodah

doi:10.33762/bagrs.2011.57188

	EFFECT OF SOME CHEMICAL COMPOUNDS, SURFACTANT MATERIALS AND COMMERCIAL DETERGENTS ON THE ACTIVITY OF LIPASE ENZYME PURIFIED FROM HEPATOPANCREASE OF CRAB Portunus pelagicus (L. 1758)
Basrah Journal of Agricultural Sciences
Article 12, Volume 24, Issue 2, 2011, Pages 177-192 PDF (0 K)
DOI: 10.33762/bagrs.2011.57188
Authors
Amar Y. J. Al-Sraji; Sabah M. H. AL-Shatty; Raodah M. Al-Ali
Abstract
This study aimed to assess partial biochemical characteristic of the enzyme purified from crab portunus pelagicus, some of the ion compounds inhibition the activity of lipase, in the presence of copper and iron ions when incubated of the enzyme with 1 and 5 mM and 5 mM manganese, while both of a calcium and magnesium ions increase in the activity of the enzyme at the concentration of 5 mM, and the results showed the activity of the enzyme was also affected by the presence of calcium ions, magnesium and manganese by a few at the concentration of 1 mM. There is no effect of the chelating agents and reduction as EDTA and 2-mercaptoethanol and Urea on the activity of lipase, when incubated it in 1 and 5 mM. Results showed an increase in the activity of lipase in the presence of surfactant materials such as Tween 20 and Triton X-100 at the concentration of 5 and 10 (V /V%), respectively, while the some materials at 10 and 5 (V /V%), 5 , 10 (V /V%) of Tween 80 , 0.1 (W/ V%) of SDS showed simple inhibition of lipase. Commercial detergents such as (Sana , Cleana , Bounx , Albana) at 0.1 mM were not affect on lipase activity.
Keywords
Portunus pelagicus

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