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Isolation, purification and partial characterization of ficin from Ficus carica latex | ||
| Journal Of Wassit For Science & Medicine | ||
| Article 1, Volume 2, Issue 2, 2009, Pages 55-66 | ||
| Authors | ||
| Ahmed D; ; ; Abdul Aal F | ||
| Abstract | ||
| This study aimed at the isolation, purification and characterization of fig latex ficin. Samples were collected from fruit joint with the fig tree. Supernatant was decanted from fig latex after centrifugation at 3000 rpm which represents crude enzyme extract. Latex content was concentrated by sucrose powder. Purification steps were carried out with ion-exchange and gel filtration using DEAE- cellulose and sephadex G-50 columns respectively. Enzyme yield and purification folds were 51.688% and 15.635 respectively with specific activity 148.336 U/mg. By using gel filtration, molecular weight of the purified enzyme was estimated to be 22.725 Da. | ||
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