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In Vitro Influence of pH and Temperature on Extracellular Enzymic Activity of Protease in Nematode-Trapping Fungi | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 34, Issue 3, 2008, Pages 14-22 PDF (0 K) | ||
| Authors | ||
| K. J. Hammadi; Dr. Ali A. Kasim; ; | ||
| Abstract | ||
| In the present study influence of pH and temperature on extracellular enzymic activity of protease on solid media were tested of six species of nematode -trapping fungi (Arthrobotrus anchonia , A.dactyloides , A.oligospora , Dactylella brochopaga , Dactyleria pyriformic , Monacrosporium eudermatum) . The extracellular enzymic activity of protease were varied according to fungal species and the value of pH and temperature . A.oligospora produced high vegetative growth in all pH values , followed by M.eudermatum , whereas D.pyriformic showed a low vegetative growth followed by A.anchonia . Nevertheless, all tested fungi showed a high vegetative growth at pH = 7.5 , followed by value 7 , whereas it's growth was weak at pH = 8.5 , no vegetative growth showed at pH = 6 . A.oligospora showed a high enzymic activity followed by M.eudermatum , a low enzymic activity was appeared in D.pyriformic . A best enzymic activity in the tested fungi produced at pH = 7.5 , followed by pH = 7 , while all tested fungi were never showed enzymic activity in pH = 6 and 8.5 . This Study showed that the M.eudermatum produced a highest growth rate in all tested temperature degrees , followed by A.oligospora , whereas D.pyriformic showed lowest growth rate , the vegetative growth in other tested fungi were varied However, the optimum temperature for vegetative growth of all tested fungi was 25 C° , followed by 30 C° , while the lowest vegetative growth was appeared at 40C° followed by 15C° . The high activity of protease showed in A.oligospora , followed by M.eudermatum , the low activity revealed in D.brochopaga, in tested temperature degrees . the 30C° showed a high enzymic activity . followed by 25C° , on the contrary , no enzymic activity appeared in all tested fungi in both 15C° and 40C° | ||
| Keywords | ||
| nematode; trapping fungi; protease; temperature; Extracellular enzymic activity | ||
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