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Extraction of phenolic compounds from wheat bran and its assessment as antioxidants | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 33, Issue 2, 2007, Pages 8-15 PDF (0 K) | ||
| Author | ||
| Ali Khudhair Jabir Al-Rikabi | ||
| Abstract | ||
| Phenolic compounds were extracted by distilled water , chloroform , ethanol , methanol ,propanol and ethyl acetate from wheat bran and determined by Folin-Ciocateau method.The antioxidative activity of the extracted compounds towards linoleic acid oxidation were determined. The extracts of distilled water and chloroform were fractionated by Dialysis and its antioxidative activity were determined. Influence of temperature and chemical treatments on stability of antioxidative phenolic compounds to both extracts were studied.The ability of both extracts to scavenging of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) were studied. The ability of chloroform extract on oxidation retardation of fish (subor)oil was determined.The extracts of distilled water and chloroform showed maximum concentration of phenolic compounds and highest antioxidative activity . Fractionation by Dialysis showed that extracts of distilled water and chloroform compounds with high molecular weight were mainly accounted for antioxidative activity . Stability of chloroform extract was higher than distilled water extract toward temperature and chemical treatments . Maximum scavenging of free radical activity of both extracts were at 100 mg/ml, however activity was the highest for the chloroform extract. Scavenging activity was being increased with time and concentrations . Inhibitory effect of chloroform extract to retard fish oil oxidation was increased with concentrations. The concentrations of 8 and 10 mg/g oil showed the highest antioxidative activity at 50 days period from storage. | ||
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