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Eeffect of NaCl and a mixture of (NaCl + CaCl2) on fresh weight, mineral content and prolin accumulation in the callus of date palm (Phoenix dactylifera L.) c.v. Barhi cultured in vitro . | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 33, Issue 2, 2007, Pages 16-21 PDF (0 K) | ||
| Author | ||
| Hussein K. Al-Kabby and Luma H. Adul-Qader | ||
| Abstract | ||
| Laboratory experiments were conducted to study the effect of different concentrations of NaCl solution (0 , 1 , 2 , 3 )% and mixture of NaCl with previous concentrations with two concentrations of CaCl2 ( 0.5 , 1)% on fresh weight, callus content of sodium , potassium , calcium ions and the amino acid (proline) .Explants used was shoot tip of date palm cv. (Barhi) cultured in vitro using “MS” medium with some additives and using NAA(naphthaleneacetic acid) and 2iP(2-isopentenyl-adenine) growth regulators for callus induction and multiplication .Results showed that increasing NaCl concentration Caused a decrease in fresh weight, calcium and potassium concentration In the primary callus whearase it cause an increase in sodium and proline cotent .Results showed also that addition of CaCl2 cause a significant increase in fresh weight a compaind with increasing concentrations of potassium, calcium and cause decreasing concentration of sodium and proline in the primary callus . | ||
| Keywords | ||
| date palm; callus; NaCl; CaCl; proline | ||
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