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Study of Antibacterial Activity of Aminoglycosidic Antibiotic Isolated From Local Strain Streptomyces sp. 1 | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 33, Issue 2, 2007, Pages 43-51 PDF (0 K) | ||
| Author | ||
| Afrodet A. Salih | ||
| Abstract | ||
| n aminoglycosidic antibiotic active against gram positive and gram negative bacteria, was isolated from the local strain Streptomyces sp. 1 isolated from soil of southern Iraq. The antibiotic was isolated as white powder with maximum yield equal to 3 gm /l at (28-30) oC. The physical and chemical properties were studied using thin layer chromatography (TLC) , IR Spectrum, UV-spectrum, melting point, solubility in organic and inorganic solvents and qualitative testes of extracted antibiotic. The antimicrobial activity of the extracted aminoglycosidic antibiotic was determined against types of standard strains of bacteria which are Staphylococcus aureus NCTC 6571 and Escherichia coli NCTC 5933. The results indicated that the isolated aminoglycosidic antibiotic has varying degrees of inhibiting the test organisms. The minimum inhibition concentration (MIC) of the aminoglycoside antibiotic was estimated by using filter disk assay against types of gram positive and gram negative bacteria. The (MIC ) of the aminoglycoside antibiotic against gram positive bacteria was ( 0.75 µg/ml. ) and was ( 7.0 µg/ml. ) against gram negative bacteria. | ||
| Keywords | ||
| antibiotic; Aminoglycoside; Streptomyces; Gentamycin; Streptomycin; Gentamycin Streptomycin | ||
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