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Isolation of leptin hormone from plasma and molecular weight estimation using bio techniques | ||
| Journal of Education and Science | ||
| Article 23, Volume 22, Issue 2, 2009, Pages 61-70 PDF (0 K) | ||
| DOI: 10.33899/edusj.2009.57654 | ||
| Authors | ||
| Nawal Thnoon Youis; Qusi Al Jalabi; Thikra Ali | ||
| Abstract | ||
| Abstract The research include the isolation of leptin hormone from plasma of female by gel filtration chromatography technique using separating column 1002Cm containing Sephadex G - 75 produced 5 bands where number 5 showed leptin concentration 0.5ng/ml. The apparent molecular weight of leptin had been determined by gel filtration chromatography technique using Sephadex G - 75, and by SDS electrophoresis, the results showed that the apparent molecular weight for the band number 5 of leptin hormone was 15750353 Dalton and 16000 Dalton respectively. | ||
| Keywords | ||
| leptin hormone; molecular weight; bio techniques | ||
|
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