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Production of Gladiolus grandiflorus by tissue culture technique | ||
| Journal of Education and Science | ||
| Article 42, Volume 22, Issue 2, 2009, Pages 324-332 PDF (369.07 K) | ||
| DOI: 10.33899/edusj.2009.57687 | ||
| Author | ||
| Khazel Ali Ameen | ||
| Abstract | ||
| We achieved an economical conclusion for Laboratory propagation of gladiolus bulbs Gladiolus grandiflorus using tissue culture techniques. This method include the use of corm slices with 8-10 millimeters thickness which were lied in contact with Murashige and Skoog solid medium supported with 0.25, 0.5, 1.0 and 2.0 mg/L of NAA or 2,4-D interaction with different concentrations (0.5, 1.0, 2.0 and 4.0 mg/L) of BA and Kinetin. The number of shoots obtained at the level of 4.0 mg/L BA combined with 1.0 mg/L of NAA was 5.1 folds while the largest number of shoots 4.9 folds were found with the kin at 4.0 mg/L with 1.0 mg/L of NAA. The later has more impact on vegetative growth and the number of shoots formed than 2,4-D. The sucrose and NAA had a great effect on the rooting of shoots and formation of corms, through the best periods of time for each of them. Rooting were 100% when used 60 g/L sucrose within 14 days, and took 56 days to produce corms. When 90 g/L of sucrose were used with 10 mg/L NAA for rooting, shoots were rooted within 11 days and corm formation 7.0 corm/shoot were accomplished after 42 days. The corms were ready for planting in the filed. | ||
| Keywords | ||
| Gladiolus grandiflorus; Tissue culture; Production | ||
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