- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
In vitro study of Methotrexate- polypeptide effect on Leishmania donovani | ||
| Journal of College of Education for Pure Science | ||
| Article 1, Volume 2, Issue 3, 2012, Pages 32-39 | ||
| Authors | ||
| Ayad Q. mahdi; Raheem J.M | ||
| Abstract | ||
| Methotrexate (MTX) has been supported by the polypeptide (insulin). the degree of MTX incorporation into polypeptide (1:0.5) the molar substitution ratio was applied in the experimental reaction . the spectrophotometers were used for characterization of MTX-polypeptide product. The in vitro coupling of MTX-polypeptide are shown to be an inhibitant of L.donavani growth. The growth rate of parasites decreased in parallel with increasing the concentration of MTX- polypeptide (0.02, 0.04 and 0.06 mg/L) & lowering as compared to the control group. The growth rate is counted during period of as long as 72 hrs. The maximum decline in growth rate was obtained in the third day at concentration of 0.06 mg/L of MTX- polypeptide. There is a significant difference (p<0.05) in replication rates of promastigotes among the different concentrations of MTX- polypeptide (mainly at 0.06 mg/L). | ||
| Keywords | ||
| Methotrexate; polypeptide; leishmania donovani; In Vitro; Insulin; replication rates | ||
|
Statistics Article View: 39 PDF Download: 9 |
||