- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Immune response to heat shock protein 60 and its relations t enteric reactive arthritis | ||
| Kerbala Journal of Medicine | ||
| Article 24, Volume 2, Issue 5, 2009, Pages 732-736 PDF (0 K) | ||
| Authors | ||
| Razak H. Ahmed; ; Mohammed hadi; Jabbar Salman Hussan | ||
| Abstract | ||
| background: according to the molecular mimicry hypothesis, heat shock protein 60 (hsp60) is among the most conserved proteins that have been implicated as triggering agents in the pathogenesis of enteric reactive arthritis (ReA). Objective: In the present work, we aimed to determine the prevalence of anti-hsp60 antibodies in sera of patient with enteric reactive arthritis. Methods: Forty-five patients with Reactive arthritis were enrolled in this study. They were 22(48.89%) males and 23(51.11%) females, the age rang was 20 - 40 year with mean (33.6 ± 10.6). All patients were outpatient visitor or hospitalized in the medical City hospital in Baghdad. They were diagnosed clinically by consultant rheumatologist with the aid of some laboratory tests such as RF, CRP, E.S.R. The patients should be sero-negative (RF-negative) and fulfill Amor and European Criteria to be included in this study. Patients were classified according to disease activity into three groups: sever moderat and mild by using (DAREA score). Thirty age and sex matched apparently healthy individual, were considered in this study as a control group. ELISA was used to detect immune response against hsp60 in the sera of each patients and controls. Wells of the micro titter plates were coated with hsp60 in coating buffer and anti hsp60 antibodies were assayed. Results: the mean age of patients was (33.6± 10.6) years and they were 23 females and 22 males with females to male ratio 1.05:1, the majority of patients 19(42.22%) present with high disease activity (sever) and 15(33.33%) patients were moderate and the remainder 11 (24.44%) were mild disease group. The result of anti-hsp60 Abs detection showed that there was highly significant difference between ReA patients and control groups. Also there was significant difference between sever, moderate and mild among ReA patients. Conclusion: we concluded that bacterial hsp60 seems to be a major target of T-cell response in enteric ReA. and cross reactivity against autologous hsp60 has been documented as a triggering of enteric ReA. | ||
| Keywords | ||
| Reactive arthritis; heat shock proteins; Development of a disease activity index for the assessment of reactive arthritis | ||
|
Statistics Article View: 180 PDF Download: 163 |
||