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Explanation of Theophylline site of action in cells of skin pathogenic fungi by inhibition cascade hypothesis | ||
| Kerbala Journal of Medicine | ||
| Article 11, Volume 1, Issue 2, 2007, Pages 248-251 PDF (0 K) | ||
| Author | ||
| AL-Janabi A. A | ||
| Abstract | ||
| background: The mode of action of theophylline has been determined in mammalian cells by three mechanisms, CAMP phosphodiesterase (PDE) inhibition, adenosine receptor antagonism and calcium mobilization, but after determined its activity against Trichophyton mentagrophytes, one of Dermatophytes fungi, therefore, it is needful to be known the effective site of action in the cells of fungi. Methods: Trichophyton mentagrophytes was cultured in Sabouraud’s glucose broth that contains 1, 3, and 5 mg/ml of theophylline. Morphological changes were noted in the treated cells when stained with trypan blue using light microscopy in addition to the measurement of the electrical conductivity to the medium which contains such cells. Result: Morphological changes were noted in Trichophyton mentagrophytes cells after incubation in a media containing 3 mg/ml of theophylline. These changes included aggregation of protoplasmic materials at the center of fungal cells (spores and hyphi). Plasma membrane permeability was not affected by this compound after measurements of electrical conductivity and staining by trypan blue stain. Inhibition cascade hypothesis, that it is proposed by the author, explained these morphological changes according to the supposed series of biochemical steps and depending on information of theophylline biochemical activities in fungal living cells. Conclusion: Inhibition cascade hypothesis tries to explain the site of action of theophylline by series of biochemical steps according to the information about its mechanism of activities in the mammalian cells. Plasma membrane is not affected by theophylline compound. | ||
| Keywords | ||
| theophylline; Trichophyton mentagrophytes; cascade; hypothesis | ||
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