Isolation of Mycobacterium Spp From Fish

S. Abbas, M.

	Isolation of Mycobacterium Spp From Fish
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 5, Issue 1, 2012, Pages 1-9 PDF (1.18 M)
Author
M. S. Abbas
Abstract
This study was designated to Isolate Mycobacteria in Fish 100 rectal swabs were collected: 28 swab from Ilisha, 1 swab from Barbus xanthopterus, 1 swab from Barbus grypus, 24 swab from Cyprinu carpio, 25 swab from Liza abu, 4 swab from Aspius vo-rax, 7 swab from Barbus Luteus, 2 swab from Barbus sherpeyi, 1 swab from Carraius auratus, 2 swab from Mugil cephitus, 5 swab from Hypophthilminthys molitrix. The swab carried on enrichment media then the swab treated with NaOH 4% for 10 mintes the swabs were cultured on special media for the mycobacteria Lowenstein- Jenson media (L J) and Tryptic soya Agar (TSA) incubated at 25 C for 8 weeks. Diagnosis of my-cobacteria was based on rate of growth, characteristics of bacterial colonies and the abil-ity of production of chromogenes and direct macroscopic examination (Acid–Fast Stain) important for Fish Tuberculosis. The results revealed isolation of mycobacterium Spp 64% from 100 Fish include: 17 isolates 60.7% from Ilisha, one isolates 100% from Barbus sharpeyi, 16 isolates 66.6% from Cyprinu carpio, 17 isolates 68% from Liza abu, 4 isolates 100% from Aspius vorax, 2 isolates 40% from Hypophthilminthys molitrix, 4 isolates 57.1% from Barbus Luteus, one isolates 50% from Barbus sharpeyi, one isolates 100% from Carraius auratus, one isolates 50% from Mugil cephitus. The result revealed that 54 isolates of mycobacteria spp grow in 3- 7 day indicate that it was from type of Rapid growing Atypical Mycobacteria and 10 isolates of mycobacteria spp grow in 8 days – 8 weeks indicate that it was from type Slow growing Atypical Mycobacteria. From this study we concluded that Fish are important sources for dissemination of my-cobacterial infections to human and animals.

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