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ISOLATION OF CYSTEINE AND METHIONINE AUXOTROPHIC MUTANTS IN SINORHIZOBIUM MELILOTI | ||
| Basrah Journal of Veterinary Research | ||
| Article 1, Volume 5, Issue 1, 2006, Pages 7-12 PDF (0 K) | ||
| DOI: 10.33762/bvetr.2006.59077 | ||
| Author | ||
| BASIL A. ABBAS | ||
| Abstract | ||
| Random 'l‘n5 mutagenesis produced 2l cysteine/methionine and 13 methionine au.'otrophic mutants of‘ Sinorliiznbimn me/1'/or/' strain Rmd20l. Linkage of Tn5 to auxotroph indicated that each mutant had a single 'l‘n5 insertion. All auxotroph showed spontaneous reversion to prototrophy and they resembled the parental strain in production ofcell surface molecule and utilization of sugars and dicarboxylic acid. | ||
| Keywords | ||
| Cysteine; Klebsiella; Rhizobial cell | ||
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