Dawwas Matter Al-Maliki, A. (2012). Isolation and Identification of Tannins from Ficus carica L. Leaves and Study of Their Medicinal Activity Against Pathogenic Bacteria. , 3(3), 96-106.
Abbas Dawwas Matter Al-Maliki. "Isolation and Identification of Tannins from Ficus carica L. Leaves and Study of Their Medicinal Activity Against Pathogenic Bacteria". , 3, 3, 2012, 96-106.
Dawwas Matter Al-Maliki, A. (2012). 'Isolation and Identification of Tannins from Ficus carica L. Leaves and Study of Their Medicinal Activity Against Pathogenic Bacteria', , 3(3), pp. 96-106.
Dawwas Matter Al-Maliki, A. Isolation and Identification of Tannins from Ficus carica L. Leaves and Study of Their Medicinal Activity Against Pathogenic Bacteria. , 2012; 3(3): 96-106.

Isolation and Identification of Tannins from Ficus carica L. Leaves and Study of Their Medicinal Activity Against Pathogenic Bacteria

JOURNAL OF THI-QAR SCIENCE
Article 1, Volume 3, Issue 3, 2013, Pages 96-106
Author
Abbas Dawwas Matter Al-Maliki
Abstract
Abstract :
In this study, tannins were isolated by reflux method using concentration sulphuric acid as
precipitating agent. The physical and chemical properties of isolated compounds, were studied such as
qualitative tests, thin layer chromatography (TLC), functional groups test and infra – red (IR) spectrum. TLC
results showed presences of two compounds have flow rate (Rf) values equal to 0.44 and 0.67 and they were
detected by using colour developers.The medicinal activity of isolated tanninic compounds, was measured
against three types of pathogenic bacteria : Staphylococcus aureus, Micrococcus roseus (Positive toward
Gram stain), and Proteus mirabilis (Negative toward Gram stain). The concentrations (25, 40, and 90 mg/ml)
recorded inhibition diameters against Staphylococcus aureus bacteria equal to (11, 13, and 14 mm)
respectively, also these concentrations recorded inhibition diameters against Proteus mirabilis bacteria equal
to (0, 0, and 8 mm) respectively whereas they didn’t show any inhibitory activity against Micrococcus roseus
bacteria. It was found that the minimal inhibitory concentration values were (25 and 90 mg/ml) against
Staphylococcus aureus and Proteus mirabilis bacteria respectively. As a conclusion, isolated tannins can be
used as herbal therapeutic substituents for treating diseases caused by Staphylococcus aureus and Proteus
mirabilis instead of antibiotics, but this work demand further clinical and pharmaceutical studies.
Keywords
Ficus carica L; Tannins; pathogenic bacteria; Medicinal activity; Minimal inhibitory concentration
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