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Purification and Characterization of alpha-amylase produced from a local Malt Barley | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 37, Issue 5, 2011, Pages 92-103 PDF (0 K) | ||
| Authors | ||
| Ghyath .H.Majeed; Ali .A.Sahi Dhia .F .Alfekaiki | ||
| Abstract | ||
| The study included , alpha amylase was extracted from malt barley local by Imidazol buffer of pH 7.4 , 0.025 M. Alpha-amylase was purified by number of steps which induced precipitation by ammonium sulphate saturated concentration was 30- 70 % and dialysis was performed against the same buffer . The result extract was passed through a column of sephdex G-100 . Purification was 20.71 fold and enzyme yield was 20.72%. Molecular weight of alpha – amylase was 40000 Dalton as estimated by SDS –Polyacrylamide electrophoresis. Optimum pH for activity and stability of enzyme was 5 and (5-7), respectively . Heat stability of enzyme increased in the presence of Ca++ ione, at a concentration of 0. 001 M , when the enzyme retained all of its activity when heated to 80 °C for 20 min while 30 % of the activity remained at 90°C for 30 min . Kinetic constants study when using starch as a substrate showed that Km was 0.55% and Vmax was 255.75 unit /ml | ||
| Keywords | ||
| Alpha amylase | ||
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