Comparative study of irradiation of blood by MS9 and Try pan blue methods on blood storage

F. Hasan, Fatehiya

	Comparative study of irradiation of blood by MS9 and Try pan blue methods on blood storage
IRAQI JOURNALOF COMMUNITY MEDICINE
Article 1, Volume 24, Issue 1, 2011, Pages 56-60
Author
Fatehiya F. Hasan
Abstract
Background: Transfusion-associated graft-versus-host disease (TA-GVHD) is a rare, but usually fatal, complication of transfusion. The risk associated with an individual transfusion depends on the number and viability of contaminating lymphocytes, the susceptibility of the patient's immune system to their engraftment and the degree of immunological disparity between donor and patient. The mainstay of prevention is gamma irradiation, which inactivates T lymphocytes whilst preserving the function of other blood cells. Objective: Comparison between the two methods of assay of MS9 and Try Pan blue assay to study the effect of radiation on blood storage Materials & Methods: Nine pints of blood had been taken from Notional Center for blood transfusion. From these pints, 255 samples were taken for test, 156 samples of blood exposed to high doses of gamma radiation of 15, 18, 20, 22, 24, 26, 28, and 30Gy. This radiation, obtained from 60Co available in Hospital of Radiotherapy and Nuclear Medicine in Baghdad, and the other 99 samples were used as a control. Blood Components are count by try pan blue in a way that nine pints of blood were utilized and from each pint several samples were used, eight of them were exposed to radiation of doses 15, 18, 20, 22, 24, 26, 28, and 30Gy, assay of try pan blue was used for counting the No. of red blood cells & lymphocyte cells whenever testing. The other pint used as a control sample before and after irradiation immediately. Results: From the results the assays by Try pan blue to count the viability of (lymphocyte cells and RBCs) was more accurate than assay by heamocounter MS9 (Haemocounter MS9 was used for automatic counts of blood cells) to know the period of the blood storage. Keywords: Irradiation, MS9, Try pan blue, Blood storage
Keywords
irradiation; Try pan blue; Blood storage

Statistics Article View: 32 PDF Download: 13