EFFECT OF BLACK SEEDS, GARLIC POWDER OR ENROFLOXACIN ON E-COLI-INDUCED-AIRSACCULITIS IN BROILER CHICKENS

Kassab, Atheer; Kamal, Nawal

	EFFECT OF BLACK SEEDS, GARLIC POWDER OR ENROFLOXACIN ON E-COLI-INDUCED-AIRSACCULITIS IN BROILER CHICKENS
iraqi poultry sciences journal
Article 1, Volume 5, Issue 1, 2010, Pages 96-110
Authors
Atheer Kassab; Nawal Kamal
Abstract
Two hundred and fifty Ross-308 broiler chicks were equally divided into 5 treatment groups (Gs). Gs 2-5 were inoculated at 22 days of age in the caudal thoracic air sac with 0.5 ml normal saline containing 105 colony forming unit of pathogenic field isolate of Escherichia coli (E.coli). G 1 kept as negative (-ve) and G 2 as positive (+ve) control (Co). G 3 and 4 were fed either 1% black seed (BS; Nigila sativa) or 1% garlic powder (GP; Allium sativum) from day 1 to slaughter at 42 days of age, respectively. One-day post inoculation (PI), G5 was given Enrofloxacin1 (En) in drinking water for 5 days. Six males and six females from each group were slaughtered 6 days PI for pathological indexes (PIdxs; 0-4). Ten days PI, 15 birds from each group were blood-sampled, from the wing vein for the following estimations: 1. Newcastle Disease (ND) ELISA antibody titers (EAbTs), 2. ND Hemagglutination Inhibition antibody titers (HIAbTs), 3. Mycoplasma galisepticum (MG) EAbTs, 4. Slide films for heterophil/ lymphocyte (H/L) ratio. At slaughter, Thymus weight (TW), Spleen weight (SW), and Bursa weight (BW)/body weight (BW) were recorded from 10 birds in each group. Birds were vaccinated against ND by coarse spray at 1, 15 and 30 days of age, and against Infectious bursal disease (IBD) in drinking water at 12 and 22 days of age. Results showed that all inoculated birds developed signs of airsacculitis second day PI. The mean PIdxs were 0, 3.66, 1.33, 1.10 and 0.58 for the Gs, respectively. Gs BS, GP and En were significantly (P≤ 0.05) differed from +ve Co, but not among themselves. There were no significant differences between the Gs, neither in ND-EAbTs nor in ND-HIAbTs. The MG-EAbTs were zero for all the Gs. Mean percentages of SW/BW increased in BS (P≤ 0.05) and GP over En. The ratios were 0.14, 0.12, 0.14, 0.14, and 0.11 for the Gs, respectively. BW /BW ratio increased (P≤ 0.05) in BS group over +ve Co and GP groups. However, there were no significant differences in the TW/BW ratio between the groups. In comparison with the –ve Co, the mean H/L ratios for BS and GP were reduced (P≤ 0.05), while those of +ve Co and En were elevated (P≤ 0.05). Mean body weights at slaughter were 2540, 2504, 2718, 2607, 2452 grams for the Gs respectively. Group BS was heavier (P≤ 0.05) than all other Gs, and group GP was heavier than +ve Co and En. There were insignificant differences in mortalities between the Gs. In conclusion, 1% black seed or garlic powder in feed protected the birds against experimentally induced E.coli infection at a similar manner as Enrofloxacin therapy in water and improved spleen weight, H/L ratio, birds’ productive performance and had better economic return.

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