SOME PROPERTIES OF A PURIFIED LYSOZYME FROM THE LATEX OF CALOTROPIS PROCERA

M. Abdul-Rahman, S.; A. Shaker, K; K. Dalaly, B.

	SOME PROPERTIES OF A PURIFIED LYSOZYME FROM THE LATEX OF CALOTROPIS PROCERA
The Iraqi Journal of Agricultural Science
Article 1, Volume 37, Issue 2, 2006, Pages 105-112
Authors
S. M. Abdul-Rahman; K A. Shaker; B. K. Dalaly
Abstract
The aim of this study was to investigate some properties of a lysozyme purified from the latex of Calotropis procera. The results of amino acids analyses of the enzyme showed that the percentage of non-polar amino acids reached 39.1%, while the percentage of acidic and basic amino acids were 18 and 15%, respectively. The enzyme contained only 184 amino acids. The isoelectric point (pi) of the enzyme was 9. The percentage of carbohydrates found to be 13.575%. The maximum activity for the enzyme was at an ionic strength at 0.03 molary. The enzyme activity was reduced due to the addition of CuCl2, MnCl2 and HgCl2 to the reaction medium, and the enzyme activity was completely inhibited when HgCl2 added to the enzyme solution, while addition of NaCl enhanced the enzyme activity. On the other hand, each of histamine, histidin and N-acetyl glucosamine act as inhibitory againist the enzyme. No inhibition was noticed on the enzyme activity when EDTA or Cysteine were added to reaction mixture. While the non-ionic detergents (SDS) caused a great inhibition in the activity of the enzyme.

Statistics Article View: 82 PDF Download: 70