EFFECT OF DILUENT SUPPLEMENTATION WITH OLIVE OIL ON SUSCEPTIBILITY TO LIPID PEROXIDATION DURING LIQUID STORAGE OF AGED ROOSTERS' SEMEN

J. AI - Daraji, Hazim

	EFFECT OF DILUENT SUPPLEMENTATION WITH OLIVE OIL ON SUSCEPTIBILITY TO LIPID PEROXIDATION DURING LIQUID STORAGE OF AGED ROOSTERS' SEMEN
The Iraqi Journal of Agricultural Science
Article 1, Volume 37, Issue 2, 2006, Pages 177-187
Author
Hazim J. AI - Daraji
Abstract
The aim of this experiment was to investigate the effects of supplementing the diluent of aged roosters' semen with different levels of olive oil on motility, viability, morphology and acrosome integrity of roosters spermatozoa after in vitro storage for up to 72 h. Semen was collected from 60 White Layer males (62 wk of age) kept in separated floor pens and randomly divided into six treatment groups (10 males in each group). Experimental groups were as follows: Tl :fresh semen, T2 : semen extended 1:1 with Al -Daraji 2 diluent (AD2D) alone, T3 - T6 :semen samples extended 1:1 with AD2D supplemented with 2 ml, 4 ml, 6 ml or 8 ml of olive oil / 100 ml of diluent, respectively. Semen samples were then stored at 5 °C for 24 h, 48 h or 72 h. There was a clear influence of diluent supplementation with olive oil on the spermatozoa motility profile; olive oil groups (T3, T4, T5 and T6) recorded the highest scores of mass activity and individual motility during all storage periods compared to Tl and T2 groups. In addition, the inclusion of olive oil into semen diluent (T3, T4, T5 and T6) gave significantly higher percentages of viable spermatozoa, normal morphologically spermatozoa and intact acrosomes irrespective of storage period. These results clearly show that supplementation the diluent of aged roosters' semen with olive oil can improve semen quality when semen samples in vitro stored at 5 °C for up to 72 h.

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