Effect of dietary supplementation with rocket salad (Eruca sativa) seeds powder on concentration of testosterone in blood serum and malondialdehyde in testis tisue of Hy – line laying breeder roosters subjected to oxidative stress induced by hydrogen peroxide

J. Al - Daraji R. H. Razuki, Hazim

	Effect of dietary supplementation with rocket salad (Eruca sativa) seeds powder on concentration of testosterone in blood serum and malondialdehyde in testis tisue of Hy – line laying breeder roosters subjected to oxidative stress induced by hydrogen peroxide
iraqi poultry sciences journal
Article 1, Volume 6, Issue 1, 2012, Pages 64-79
Author
Hazim J. Al - Daraji R. H. Razuki
Abstract
This study was conducted to evaluate the effect of adding different levels of rocket salad seeds powder to the diet on semen traits of roosters subjected to oxidative stress induced by hydrogen peroxide. A total of 60 Hy – line laying breeder roosters 57 weeks old were used in this study. Roosters were randomly distributed on 5 treatments with 3 replicates each. Each replicate constituted of 4 roosters (12 roosters for each treatment). Experimental treatments were as following: T1: Males fed control diet and normal water, T2: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.25 ml hydrogen peroxide (0.5%) / litter of water, T3: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.5 ml hydrogen peroxide (0.5%) / litter of water, T4: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 1 ml hydrogen peroxide (0.5%) / litter of water, and T5: Males fed control diet and drink water supplemented with 1 ml hydrogen peroxide (0.5%) / litter of water. Males were treated with hydrogen peroxide (6%) and rocket salad for 12 weeks starting from 59 week of male ages. Results indicated that water supplementation with hydrogen peroxide without adding rocket salad powder to the diet of roosters (T5) resulted in significant decrease in the concentration of testosterone hormone in blood serum as copared with T1, T2, T3 and T4 during 8 and 12 weeks of experiment and significant increase in concentration of malondialdehyde in testis tissue in comparison with T1, T2, T3 and T4. However, supplementing the diet of roosters subjected to oxidative stress induced by hydrogen peroxide with rocket salad seeds powder (T2, T3, and T4) resulted in significant increase with relation to concentration of testosterone hormone in blood serum and significant decrease in concentration of malondialdehyde in testis tissue as compared with T5 group. In conclusion, adding rocket salad powder to the ration of roosters could limit to a considerable extent the damages on reproductive system that originate from oxidative stress.

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