Separation and Determination of Preservative Methylparaben in Insulin Preparations Using Gas Chromatography

Mohammad Yaseen, Suhair; E. Habboush, Alberteen

doi:10.30684/etj.30.13.9

	Separation and Determination of Preservative Methylparaben in Insulin Preparations Using Gas Chromatography
Engineering and Technology Journal
Article 1, Volume 30, Issue 13, 2012, Pages 2285-2294 PDF (239.9 K)
DOI: 10.30684/etj.30.13.9
Authors
Suhair Mohammad Yaseen; Alberteen E. Habboush
Abstract
In this search an improved gas chromatographic method was used for identification and typically quantitated for methylparaben(mp) which is used as preservative in insulin preparations.it was involved the optimal conditions for extraction mp from aqueous samples with ethyl acetate ,chloroform ,and toluene as extractants,then analyzed by gas chromatography(GC). Effects of each of extractants polarity, aqueous samples pH, phases (organic to aqueous) ratio, mixing time, polarity of stationary phases, and column temperature on efficiency of separation and determination of mp. were studied in this work. Synthetic sample which contained the same contents as a life sample was tested to omit interferings of other drug additives on analysis results. The extraction of mp. was (90-91)% with one batch of ethyl acetate and (99- 100)%with two batches of ethyl acetate at pH medium(1.5-2).also the non-polar stationary phase(OV-101) is the best choice for determining mp by GC. The concentration of mp. in insulin preparations was within the acceptable range(1.003mg/ml). The relative error (R.E) was ± (0,39-1.49)% and relative standard deviation(R.S.D) was (1.21)% for life samples.
Keywords
methylparaben; extraction; preservatives

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