EVALUATION OF THE EFFICIENCY OF FIVE MEDIA FOR FUNGAL PROPAGATION OF FIVE DODDER PATHOGENIC FUNGI AND STUDY THEIR SHELF LIFE

S. Juber, K.; A. Al-Rawi, F.; Y. AL-Hitar, M.

	EVALUATION OF THE EFFICIENCY OF FIVE MEDIA FOR FUNGAL PROPAGATION OF FIVE DODDER PATHOGENIC FUNGI AND STUDY THEIR SHELF LIFE
The Iraqi Journal of Agricultural Science
Article 1, Volume 36, Issue 6, 2005, Pages 77-84
Authors
K. S. Juber; F. A. Al-Rawi; M. Y. AL-Hitar
Abstract
This study was carried out to evaluate the efficiency of five different media to increase the viability and affectivity of some dodder pathogenic fungi. Results of the efficacy test for five different media to preserve pathogenic fungi to dodder plants showed the ability of all media to keep the viability and affectivity of the testing fungi to infect dodder stems after 30 days of preparation, the averages of the percentage of disease severity ranged between 64.44 % for the MY9 isolate and 85.78 % for the MY57 isolate. Two media (groat corncob and peatmoss) showed the highest increase in percentage of disease severity (74.17 % and 72.22 %, respectively), while the least was alginate medium at 28.89 %. The results of the tests in glass house to evaluate the efficacy of the different media to infect dodder plants showed that all the testing fungi isolates infected dodder plants except MY57, the most virulent isolates were MY5, IV1Y9, MY 13, and MY33 which gave high disease severity of 66.67 % , 66.67 % , 69.99 % , 69.99 % , respectively . Also appeared significant differences among the different media, the peatmoss and groat corncob gave the highest percentage of disease severity ( 62.84 % for each of them ), followed by the Stabileze and wheat flour with kaolin media which gave an average of 47.63 % and 45.24 %, respectively. The results of the fungal preparation sheif life experiment showed that, the peatmoss medium showed high efficient in average of colony forming units 2.2 X 10s CFU/gram followed by the wheat flour with kaolin medium at an average of 8.2 X 107 CUF/gram, and groat corncob formulation at average of 7.6 X 107 CFU/gram, while the number of colony forming units was reduced during the storing period, when it reached to 94.57 %, 86.8 % after three and six months of storage, respectively.

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