Study of Toxicity and Pathogenicity of Aflatoxin B1 and G1 in Mice

N. Yassein, Sh.; R. Zghair, Z.

	Study of Toxicity and Pathogenicity of Aflatoxin B1 and G1 in Mice
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 5, Issue 1, 2012, Pages 23-31 PDF (1.24 M)
Authors
Sh. N. Yassein; Z. R. Zghair
Abstract
The results of this investigation had demonstrated the production of aflatoxins from Aspergillus flavus and study the toxicity and pathological effect in mice. Aflatoxin B1 and G1 were produced by two methods. (Corn method and YES method). The concentration of aflatoxin B1 was 2 ppm while aflatoxin G1 was 5.25 ppm by using TLC and HPLC. Three groups of animals (mice) were taken. First and second groups treated with aflatoxin B1 and G1 respectively by (25µg/0.2 ml olive oil/animal/day) for 21&30 days. This study showed that aflatoxin G1had higher effect from B1 on the liver than kidney, while aflatoxin B1had higher effect from G1 on the kidney than liver .This result was confirmed by biochemical tests for liver enzymes and kidney salts. So, the result of liver enzymes in serum of mice treated with aflatoxin G1revealed a significant increase (p<0.05) in GOT, GPT and ALP at 30 days of experiment comparing with other groups. Whereas the result of kidney enzymes in serum of mice treated with aflatoxin B1 revealed a significant increase (p<0.05) in urea, creatinin and uric acid at 30 days of experiment comparing with other groups. Therefore, we could conclude that aflatoxines have acute toxicity effect in target organs of mice that treated with short period (30 days) and may develop to induce tumors when treated for longer period (6 months).

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