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ISOLATION AND IDENTIFICATION OF THE CAUSAL AGENT OF CHARCOAL ROT DISEASE ON BEAN AND EVALUATION OF SOME BIOCONTROL AGENTS UNDER LABORATORY CONDITIONS | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 43, Issue 0, 2013, Pages 34-43 | ||
| Authors | ||
| A.A.H. Matlob; K.S. Juber | ||
| Abstract | ||
| This study was conducted to isolate and identify the causal agent of charcoal root rot disease on bean in Babylon governorate, and to examine the antagonistic ability of some biocontrol agents against the disease. The results of isolation and identification showed that Macrophomina phaseolina was present in these samples in a frequency of 3.8-76.3%., the fungus was identified up to the species level based on its cultural and morphological characteristics. A preliminary test of pathogenicity for M. phaseolina isolates showed that the isolates were differed in their effect on cabbage seed germination. The isolates MP-2B ،MP-3، MP-5 prevented seeds germination completely, compared with control (93.33 %). Results also showed that pathogenic isolates affected bean seeds germination percentage, which ranged between 0.0-17.5 % compared to 87.5% in control treatment (without pathogen). Four isolates of Azotobacter chroococcum were isolated. A. chroococcum caused significant inhibition in growth (25.25-100.0%) of M. phaseolina. T. harzianum was highly antagonistic against pathogenic isolate of M.phaseolina on PSA media. | ||
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