DETECTION OF FUSARIUM GRAMINEARIUM  IN MAIZE  SEEDS AND DETERMENATION OF ISOLATES PRODUCED TOXIN AND EVALUATING THE ACTIVITY OF SOME COMPOUNDS AGAINST THE FUNGUS ON CULTURAL MEDIA

Z. Hussein, H.; K. Slomy, A.

	DETECTION OF FUSARIUM GRAMINEARIUM IN MAIZE SEEDS AND DETERMENATION OF ISOLATES PRODUCED TOXIN AND EVALUATING THE ACTIVITY OF SOME COMPOUNDS AGAINST THE FUNGUS ON CULTURAL MEDIA
The Iraqi Journal of Agricultural Science
Article 1, Volume 43, Issue 0, 2013, Pages 95-102
Authors
H. Z. Hussein; A. K. Slomy
Abstract
This study was conducted to delect the fungus Fusarium graminearum in corn cereals, the capacity of fungus isolalis to product zeralenon mycotoxion, and the activity of some compounds against fungal growth on culture media. Samples of corn seeds from; Babel, Baghdad, Nineue, and Waseete governorate were collected. The cereals were surface sterilized in sodium hypochlite with sterilized distilled water. The cereal were dried on filter paper and distributed on potato sucrose Agar (PSA) medium in petriduhes of 9 cm diameter (10 seed / plate). The petridishes were incubated at 25 ± 2ºc for 5 days the fungi grow were identified to the genus. Results showed the presence of Fusarium graminearium with many other fungi including, Mucor spp. Alternaria spp. Penicillium spp. Aspergillus niger, A. flavus, Penicillium spp. Rhizoctonia spp. Trichoderma spp. assouatid with corn cereal. It was found that 9 of 13 isolatis of F. graminearum produced zeralenone mycotoxin. The addition of urea at 5 %, Fylax at 0.16 % to the cultural medium inhibited completely the fungal growth (100 % inhibition) . The magnetic and normal water caused an inhibition of 30.1 and 10.8 % to fungal growth respectively. No effect on the fungal growth was observed by activated bentonit to, kaolenite and Sacchromyas cerevistae powder extract.

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