Antibacterial Effect of Seed Extracts of Cardamom (Elettaria cardamomum) against Staphylococcus aureus and Proteus mirabilis

F. Salah Akrayi, Ro

	Antibacterial Effect of Seed Extracts of Cardamom (Elettaria cardamomum) against Staphylococcus aureus and Proteus mirabilis
Tikrit Journal of Pure Science
Article 1, Volume 17, Issue 2, 2012, Pages 14-18
Author
ro F. Salah Akrayi
Abstract
In this study, twenty isolates of Staphylococcus aureus and twenty others of Proteus mirabilis were collected from Hawler Teaching hospital during the period 10th January/2008 to 10th March/2008 in Erbil city-Iraq. The isolates were identified according to cultural, morphological, and biochemical properties. The susceptibility of isolates to antibiotics (Cefixime, Cefotax, Ciprophloxacin and Doxycillin) was tested, the resistance results for them, were (Cefixime, Cefotax and Doxycillin), and (Ciprophloxacin and Doxycillin) for S. aureus and Proteus mirabilis respectively. The antibacterial effects of seed extracts of cardamom (Eltttaria cardamomum) on more resistant isolates of both species were examined by well diffusion technique, all extracts have antibacterial property but methanolic extract was better than ethanolic and aqueous extracts; MIC and SMIC were determined, and used as curing agents to eliminate the antibiotic resistance genes and removing the swarming phenomenon in case of (Proteus mirabilis). It cleared from this study that methanolic extract was better than ethanolic and aqueous extracts where the curing percentage was (3.33%-70%) while the curing percentage of ethanolic extract was (3.33%-66.6%) which it was better than aqueous extract (0.00%-3.33%) in Staphylococcus aureus, while in Proteus mirabilis the results of plasmid curing was very weak where methanolic extract cured only 3.33%.
Keywords
antibacterial activity; Elettaria cardamomum; Staphylococcus aureus; Proteus mirabilis; well diffusion technique; plasmid curing; antiswarming; MIC

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