- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Evaluation the effect of the use Immunoglobulin IgY immunity of infected chickens with the Eimeria tenella parasite | ||
| Journal of University of Anbar for Pure Science | ||
| Article 7, Volume 6, Issue 1, 2012, Pages 19-28 PDF (597.28 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2012.62116 | ||
| Authors | ||
| THAER A. S. AL-ALOOSI1; TAWFIQ A. AL-ALOOSI2; SHEHAB A. ALJEBORY3 | ||
| 1University of Anbar - College of Science | ||
| 2Tikrit University - College of Veterinary Medicine | ||
| 3Tikrit University - College of Education | ||
| Abstract | ||
| Been obtained for the isolation of local Eimeria tenella by taking the Cecca of 850 samples from chickens slaughterhouses in the city of Ramadi, and after purification was diagnosed through the shape and size of the parasite and the infection , obtained the challenge strain by strengthening the isolation in Chicken type Ross 308 five times, injected challenge strain in laying hens Type Luhman under the skin and muscle of the chest and thigh after mixing with Freund's incomplete oil adjuvant solution, then get IgY from egg yolk, which began collected three days after the third dose, detection of IgY by using singular and double immunodiffusion test, use the IgY against Eimeria tenella in the treatment concentration of 1/4 with a dose of 5x 104 of the challenge strain and three replicates of treatment, showed the experiment results of the treated chicks with IgY, three days after giving the challenge dose has given the proportion of immunity amounted to 51.12%, while the results given the challenge dose with a solution of IgY given the proportion of immunity amounted to 75.55%, and finally give the challenge dose after giving the solution IgY in three days gave the proportion of immunity amounted 88.88% without any damage in chicks treated in the experiment compared with the control treatment of positive and which gave the proportion of immunity amounted 95.55% compared to control negative, which gave the proportion of immunity amounted 17.78%, as demonstrated by the study of statistical significant differences at the level of probability of 0.05 for all transactions except for the first treatment with the treatment of five . | ||
| Keywords | ||
| IgY; Eimeria tenella | ||
| References | ||
|
1- Kheysin, Y.M. (1972). Life cycles of coccidia of domestic animals. University Parck Press, Baltimore, London, Tokyo. 3th Ed
2- Crurrent, W. L.; Upton, S. J. and Long, P. L. (1990). Taxonomy and life cycles. In:Long, P. L. (ed). Coccidiosis of man and domestic animals. Boca Raton, Florida. CRC Press. pp. 1-16.
3- Trees, A. J. (1990). Parasitic diseases. In: Jordan, F. I. W. (ed).Poultry Diseases. 3rd ed. English language book. Society, Bailliere, Tindall, pp.226-233.
4- Shiotan, N.; Baba, E.; Fukata, T.; Arakawa, A. and Nakanishi, T. (1992). Distribution of oocysts Sporocysts of Eimeria tenella and Eimeriamaxima in the digestive tract of chicken. Vet. Parasitol., 41:17-22.
5- Allen, P. C. and Fetterer, R. D. (2002). Recent advances in biology andimmunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clin. Microbiol. Rev., 15: 58-65.
6- الصفار، ربى احمد شوقی عبد الوهاب (2001). الکفاءة التمنیعیة لطفیلی الاکریات المضعفة بأشعة کاما فی دجاج اللحم، رسالة ماجستیر - کلیة الطب البیطری جامعة بغداد، العراق. 7- Keri Marshall, N.D. (2004). Therapeutic Applications of Whey Protein AAltern. Med. Rev.,9(2):136-156).
8- Havenstein , G.B., P.R. Ferket , and B.T. Larson ,(1994). Growth ,livability and feed , conversion of 1975 vs. 1991. broiler fed typical 1957 and 1991 broiler diets poultry Sci. 73:1785-1794.
9- Qureshi , M.A. , and K. E. Anderson ,. (1995). Comparison of immunologic functions of random bred verus present day SCWL. Laying hen strains. poultry science association. 48th. annual meeting. Abs.No.176 10-El.Behairy, A.M. (2005): Immuno-characterization on some Eimeria spp. Infecting chicken in Egypt. M.V.Sc. Thesis Fac. Of vet. Med.,CairoUniversity.11-Bowman, D.D. and Lynn, R.C. (1995) George's Parasitology forveterinarians, Philadelphia, London, Toronto, Tokyo. WB Sanders Company.2th Ed. pp297.
12-Edagar, S.A. (1955). Sporulation of oocysts at specific temperatures and notes on the prepatent period of several species of avian coccidiosis. J. Parasitol. 41:214-216.
13-Ibrahim, A.L.; Arafa, E.A. and Msahlabe, A.A. (1997). Study onpathogenicity and immunoginicity of irradiated sporulated intestinal Eimeria oocysts in chickens. Ass. Vet. Med. J. 37:133-140.
14-Jeffers, T. K. (1978). Eimeria tenella sensitivity of recent field isolants to monensin. Avian Dis., 22: 157-161.
15-Davis, L.R. (1973). Techniques. In: The Coccidia. Ed. by:Hammond, D.H. and Long, P.L. Baltimore. Butter worth, London, University Park Press.Pp.411-458.
16-Jorgensen, W.K.; Stewart, N.P.; Jeston, P.J.; Molloy, J.B.; Blight, G.W. andDalgliesh, R.J. (1997). Isolation and pathogenicity of Australian strains ofEimeria praecox and Eimeria mitis Animal Research Institute, Moorooka;Queensland, 4105. Pp10-18.
17-Al-Attar, M.A. (1981). Factors effecting the pathogenesis Eimeria necatrix infections in chicken. PhD. Thesis University of Guelph. Canada.
18-Calnek, B.W.; Barnes, H.J.; Beard, C.W.; McDougald, L.R. andSaif, Y.M.(1997). Disease of poultry. 10th ed. Mosby, Wolf. pp.865-878.
19-Edagar,S.A.(1992):Field diagnosis of coccidiosis in chickens. Agri- Bio Corporation.
20-Amer,M.M.; Awaad, M.H.H; El-Khateeb,R.M.; Abu-Elezz,N.M.; Said, S.A ; Ghetas, M.M. and Kutkat, M.A :(2010). Isolation and Identification of Eimeria from Field coccidiosis in chickens. Journal of Amrican science. (2010); 6(10).
21-Morehouse, N. F. and Baron, R. R. (1970). Coccidiosis: Evaluation of coccidiostats by mortality, weight gains and fecal scores. Exp. Parasitology 28:25-29.
22-جوامیر نهاد محمد علی عبد الله (1996).استخلاص ودراسة فعالیة بروتینات المناعة من صفار بعض الطیور الداجنة. رسالة ماجستیر. کلیة الزراعة، جامعة بغداد. 23-Hansen, P.; Scoble, J.A.; Hanson, B.; Hoogenraad, N.J.(1998).Isolation and purification of immunoglobulins from chicken eggs using thiophilic interaction chromatography. J.Immunol.Meth., 215:1-7.
24- polson ,A. (1990). Isolation of IgY from the yolks of eggs bypolyethylene glycol 6000 procedure. Immun Invest. 19:253-258.
25- Rodiger , S. ; Michael , E. ; Christian , S. (2000). Chicken egg yolk antibodies ( Production and Application ).chapter 4 ( Isolation of IgY from yolk ). eds,68-85 , Sub protocol 5, Springer Lab. Manual
26- Javier, F. ; Brito-De, E. and Torrestiana, B.(2010). Purification of egg yolk immunoglobulin (IgY) by Ultrafiltation : Effect of pH , Ionic Strength, and Membrane Properties. J. of Agri. and Food Chem. 2010,58:187-193.
27- Diana , P.; Pablo, A.C.;Esteban , G.C.; Bjorn,B. and Rudiger ,S. (2010). IgY Technology:Extraction of Chicken Antibodes from Egg Yolk by Polyethylene Glycol (PEG) Precipition. vedio internet.
28- Johnston, A. and Thorpe, R. (1987). Immunochemistry in Practice. 2nd ed. Blackwell scientific publications , London , England.
29-Lee, S. H. , Lillehoj ,H.S., Park , D.W. , Jang , S. I. Morales, A. , Garcia , D. , Lucio, E. , Larios, R. , Victoria , G. , Marrufo , D. Lillehoj , E. P. (2009 a). Protective effect of hyperimmune egg yolk IgY antibodes against Eimeria tenellaand Eimeria maxima infections. Veterinary Parasitology. 163: 123- 126.29-Lee ,S. H., Lillehoj, H. S., Park , D. W., Jang ,S. I., Morales, A., Garcia , D., Lucio, E., Larios, R., Victoria ,G., Marrufo, D., and Lillehoj, E. P.(2009 b). Induction of passive immunity in broiler chickens against Eimeria acervulina by hyperimmune egg yolk immunoglobulin Y. Poultry Science 88:562–566.
30- Little , T. M. and Hills. F. J. (1972): Statistical Methods agricultural research. Agricultural extension. University of California.
31-Watts, A.M. and Kennedy, R.C. (1999). DNA vaccination strategies against infections disease. Int.J. of Parasitol. 29: 1149-1163.
32- Long , P. and Gruber , A.( 2005). Proceedings of The IXth International Coccidiosis Conference. chapter 1. Universidade de São Paulo (USP) – Brazil.
33- Danforth, H.D. (1997). Use of live oocysts based vaccines in a vain coccidian control. In: Control of coccidiosis into next millennium. Ed byShirley, M.W.; Tomley, F.M. and Freeman,B.M. Institute for animal health, Compton, New Burg Berks. Pp. 95-96.
34- Armentero MT, Levandis G, Nappi G, Bazzini E, Blandini F (2006),"Peripheral inflammation and neuroprotection: systemic pretreatment with complete Freund's adjuvant reduces 6-yyhydroxydopamine toxicity in a rodent model of Parkinson's disease", Neurobiol. Dis. 24 (3):492–505, doi:10.1016/j.nbd. 2006. 08.016, PMID 17023164.
35- Jenkins, M.C. (2004). Control of avian coccidiosis: drug & Vaccines. Miscellaneous publishing information Bulletine, Feed information. News Service, P.3
36- Kim , W.K and Patterson ,P.H..(2003).Production of an egg yolk antibody specific to microbial uricase and its inhibitory effects on uricase activity. Poultry Science ,2003,82:1554-1558. 37- Mary, Haak-Frendscho. (1994). Why IgY? Chicken Polyclonal Antibody, An Appealing Alternative. Promega Notes Magazine Number 46, p.11 | ||
|
Statistics Article View: 526 PDF Download: 246 |
||