- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
DETECTION OF DNA REPAIR SYSTEMS OF THE BACTERIUM PSEUDOMONAS AERUGENOSA | ||
| Journal of University of Anbar for Pure Science | ||
| Article 10, Volume 6, Issue 1, 2012, Pages 29-34 PDF (341.8 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2012.62279 | ||
| Author | ||
| KHALAF JASIM MOHAMMED* | ||
| University of Anbar - College of Science | ||
| Abstract | ||
| The bacterium Pseudomonas aeruginosa was irradiated with ultraviolete light with wave length ( 254 nm ) for different periods ( 50 , 100 , 150 , and 200 sec ) . part of irradiated bacterial culture was exposed to sun light and the other part was kept in the dark . The survivors of the cells exposed to the sun light was more than in the dark and this ensure possessing the bacterium photoreactivating repair system To investigate the excision repair system, the minimal inhibitory concentration ( MIC)of caffeine against bacteria was studied by exposing the bacterium to different concentrations of caffeine ( 10 , 15 , 20 and 25 mM ) and the MIC was 20 mM , then the bacterium was exposed to different doses of U.V. light in the presence of caffeine and the study ensured the survivors of the cells in the medium with caffeine was less than the medium with absence of caffeine and this refers to possess the bacterium excision repair system. To detect the recombination repair system , the bacterium was exposed to the concentrtions 0.1 ,0.2 , 0.3 , 0.4 µg / ml of acrivlavine and the MIC was 0.3 µg / ml , then the bacterium was exposed to different doses of U.V. light in the presence of acrivlavine . The survivors of the cells in the medium with acrivlavine was less compared with the absence of acrivlavine and this indicate possessing bacterium recombination repair system . To study SOS repair system the bacterium was mutated with direct mutagens represented with nitrous acid and indirect mutagens represented with U.V. light to isolate rifampicin and chloramphenicol mutants. The study ensured the sensitivity of bacterium for mutagenesis then possessing SOS repair system . | ||
| Keywords | ||
| DNA; Pseudomonas aerugenosa | ||
| References | ||
|
[1] Lodish H, Berk A, Matsudaria P,Kaiser CA,Krieger M,Scott MP,Zipursky SL,Darnell J.(2004).Molecular Biology of the Cell, p963.WH Freeman : New york,NY.5th ed.
[2] Browner WS,Khan AJ,Ziv E,Reiner AP,Oshima J,Cawthon RM,Hsueh WC,Cummings SR.(2004)-Thegenetics of human longevity.AmJ Med 117 (11): 851-860.
[3] Braig M,Schmitt CA.(2006).Oncogene-induced senescence:putting the brakes on tumor development.Cancer Res 66:2881-2884.
[4] Roulston A,Marcellus RC,Pranton PE.(1999).Viruses and apoptosis.Annu.Rev.Microbiol.53:577-628.
[5] Toshihiro Ohta,Shin-ichi Tokishita,Kayo Mochizuki,Jun Kawase,Masahide Sakahira and Hideo Yamagata(2006).UV sensitivity and mutagenesis of the extremely thermophilic eubacterium Thermus thermophilus HB27,Genes and Environment;Vol.28,No.2:p.56-61.
[6]Sancar A.(2003).Structure and function of DNA photolyase and cryptochrome blue light photoreceptors.Chem Rev 103(6):2203-2237.
[7] Watson JD,Baker TA,Bell SP,Gann A,Levine M,Losick R.(2004).Molecular Biology of the Gene.ch.9 and 10.Peason Benjamin Cummings;CSHL press.5th ed.
[8] Volkert MR.(1988)Adaptive response of Escerechia coli to alkylation damage.Environ Mol Mutation 11(2):241-255.
[9]Budman J,Chu G.(2005).Processing of DNAfor nonhomologous end-Joining by cell free extract-EMBOJ.;24(4):849-860.
[10] Wilson TE,Grawunder U,and Lieber MR.(1997).Yeast DNA ligase IV mediates microhomologous DNA end joining .Nature 388، 495-498 .
[11] Boulton SJ,Jackson SP.(1996).Saccharomyces cerevisiae ku 70 potentiates illigitimateDNA double- strand break repair and serves as a barrier to error – proneDNA repair pathways. EMBOJ.15(18):5093-5103.
[12] Al-Dolaimi KJ.(1997)On the molecular biology of a novel halotolerant bacterium (Micrococcus sp.).M.Sc. thesis . Al – Nahreine Univ.
[13] Fong K, andBockrath RC.(1979).Inhibition of Deoxy ribo nucleic acid repair in Escherechia coli by caffeine and acriflavine after ultraviolet irradiation.J.Bacteriol.,:671-674.
[14] Al-Bakri GH,Umran MA.(1994).Mutagenesis of a novel halotolerant bacteria (Micrococcus sp.) using ultraviolet light and N-Methyl-N-nitro-N-Nitrosoguanidine.Iraqi Journal of Microbiology,Vol.6(2):55-64.
[15]الدلیمی، خلف جاسم محمد.(2007). تطفیر بکتریا Pseudomonas aeruginosaباستخدام حامض النتروز. مجلة جامعة الانبار للعلوم الصرفه. المجلد الاول .العدد الثا لث .ص 45-50. [16]Bakkenist CJ,Kastan MB.(2003).DNA damage activates ATM through intermolecular autophosphorylation and dimmer dissociation.Nature 421(6922):499-506.
[17]Wei, Qingyi;Lei Li David Chen(2007). DNA repair, Genetic Instability, and cancer.World Scientific.
[18] Moseley BEB.(1983)Photobiology and radiobiology of Micrococcus (Deinococcus) radiodurans. Photochemical and photobiological review,7:223-274.
[19] Janion C.(2001).Some aspects of the SOS response system-acritical survey.Acta Biochim Pol.48(3):599-610.
[20] Erill I,Campoy S,Barbe J.(2007).Aeons of distress:an evolutionary perspective on the bacterialSOS response.FEMS Microbiol Rev.31(6):637-656. | ||
|
Statistics Article View: 308 PDF Download: 231 |
||