EVALUATION THE EFFICIENCY OF SOME PLANT EXTRACTS IN REDUCTION OF CORN SEEDS CONTAMINATION WITH FUMONISIN B1

A. Hamad, Ahmad; Akif, R.

	EVALUATION THE EFFICIENCY OF SOME PLANT EXTRACTS IN REDUCTION OF CORN SEEDS CONTAMINATION WITH FUMONISIN B1
The Iraqi Journal of Agricultural Science
Article 1, Volume 43, Issue 0, 2013, Pages 39-47
Authors
Ahmad A. Hamad; R. Akif
Abstract
The objective of the study is to evaluate the activity of some plant extracts for inhibition of Fusarium verticillioides and reduction the contamination of corn grains with Fumonisin B1 (FB1). The concentration of mycotoxin in corn grains was determined by High Performance Liquid Chromatography (HPLC). Alcoholic and aqueous extracts of Green cress, Harmal and Fenugreek seeds were prepared , and their efficiency for Fusarium verticillioides growth inhibition was tested at 1, 3, 5% in PDA cultured media, and at 5% for reduction the toxin in corn grains. Results showed that all the grain sample contained FB1 at 342.47–661.34ppm. The aqueous and alcoholic extracts of Green cress showed the higher inhibition of fungus growth on PDA. 47.6% and 50.91% respectively compared with 16.16%, 12.16% and 23.08%, 33.83% with aqueous and alcoholic extracts of Fenugreek and Harmal respectively. It was found that the concentration 5% for the two extracts was more efficient in fungus growth inhibition 52.2%, 57.50% with green cress extract, followed by the alcoholic extract of Harmal 48.50% for the same concentration. The extracts also showed activity in mycotoxin reduction produced by the fungus with higher reduction with Harmal seeds alcoholic extract at inhibition percentage of 25.83% followed by alcoholic extract of Green cress seeds 22.90%.

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