PROPAGATION OF STRAWBERRY VIA IN VITRO ADVENTITIOUS SHOOT FORMATION TECHNIQUE

A. Ibrahim, M.; A. Al-Taha, H.; A. Saaid, Z.

	PROPAGATION OF STRAWBERRY VIA IN VITRO ADVENTITIOUS SHOOT FORMATION TECHNIQUE
The Iraqi Journal of Agricultural Science
Article 1, Volume 44, Issue 1, 2013, Pages 69-80
Authors
M. A. Ibrahim; H. A. Al-Taha; Z. A. Saaid
Abstract
This study was carried out at Tissue Culture Laboratory, College of Agriculture - University of Basrah, during the period from 2010-2012 to investigate the propagation of strawberry(Fragaria ananassa Duch.) Albion by in Vitro adventitious shoot formation. Results showed that MS medium supplemented with 2.0 and 2.5 mg/l BA + 0.2 mg/l. NAA gave highest rate of shoot number reached 23.00 and 21.67 shoots respectively. While BA concentration of 0.5 mg/l. + 0.2 mg/l. NAA gave lowest rate of shoot number (9.67 shoots). The results were reverse when kinetin replaced with BA for the same medium components of multiplication, they reached 8.33, 2.33 and 3.00 shoots at 0.5, 2.0 and 2.5 mg/l. kinetin respectively. The concentration 0.5 mg/l. of BA gave highest rate of shoot length reached 4.33 cm whereas the 2.5 mg/l. of BA gave lowest rate of shoot length (0.67 cm). The similar results showed when kinetin replaced with BA for the same medium components of multiplication, they reached 3.00 and 0.67 shoots at 0.5 and 2.5 mg/l. Kinetin respectively. Results showed that rooting medium supplemented with 1 mg/l. IBA gave high rooting percentage of shoots (100%) and produced high rates of root number and length reached (3.00 root/plantlet and 3.67 cm) respectively. Finally, the plantlets were acclimatization by culture in mixture of river soil + peat moss (1:1) in growth room at conditions: 16 hrs. light and 8 hrs. dark and 27 ± 2 °C temperature. All plantlets were survived when transferred.
Keywords
In vitro; Multiplication; benzyl adenine; Kinetin; IBA; Plantlets

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