PRODUCTION OF POLYGALACTURONASE FROM THERMOSTABLE MOLD ASPERGILLUS NIGER  2-OPTIMIZATION THE PRODUCTION BY SOLID STATE  FERMENTATION

O. Muhyalddin, Mohammed; A. Chechan, Rukaibaa

	PRODUCTION OF POLYGALACTURONASE FROM THERMOSTABLE MOLD ASPERGILLUS NIGER 2-OPTIMIZATION THE PRODUCTION BY SOLID STATE FERMENTATION
The Iraqi Journal of Agricultural Science
Article 1, Volume 44, Issue 1, 2013, Pages 106-113
Authors
Mohammed O. Muhyalddin; Rukaibaa A. Chechan
Abstract
This part of study was aimed to investigate the optimal condition of production the enzyme Polygalacturonase from Aspergillus niger TP4 by solid state fermentation using different wastes available locally. It was found that the higher level of production could be achieved on wheat bran and sunflower head extract moistened with water at a ratio 3:1 with an initial pH 5 inoculated with 107 spores/gm during an incubation time of 4 days at 40 C0.The crud enzyme was extracted by adding 15ml of water to each flask followed by filtration and centrifugation at 1000 for 15 min the resultant extract was used for enzyme assay polygalacturonase activity was assay by measuring reducing groups The productivity extract was used for enzyme assay polygalacturonase activity was assay by measuring reducing groups The productivity under these conditions was 325.45 unit/gm using wheat bran and 242.28 unit/gm using sunflower head with an increase estimated about 124.7% and 120.8% respectively in relationship with productivity before of the optimization Maximal levels of enzyme activityes were achieved upon growing upon growing the culture in amedium containing wheat bran after 4 days of incubation at temperature of 40 C0 with pH 5 .
Keywords
Aspergillus niger; polygalacturonase; agrowastes; Pectin; fermentation

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