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ISOLATION AND DETERMINATION OF PROTEIN FRACTIONS OF Trichophyton mentagrophytes Var.erinacei USING GEL FILTERATION CHROMATOGRAPHY | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 38, Issue 4, 2012, Pages 1-9 PDF (0 K) | ||
| Author | ||
| Afrah Z. Risan | ||
| Abstract | ||
| The protein in broken-cell extract from Trichophyton mentagrophytes var.erinacei was isolated and fractionated using sephadex G-100 gel filtration technique. On ultra-violet absorption of the isolated fractions was measured at 280 nm. the molecular weight of these fractions was calculated and found to be four fractions ranging :102,000,220,000,425,000 and 610.000 Daltons. Amino acid analyses showed a predominance of the following: glutamic acid ,glycine ,proline,valine,methionine and cyctine.In conclusion the information derived may be useful in the classification of Trichophyton species. | ||
| Keywords | ||
| Protein fraction; Trichophyton mentagrophytes Var; erinacei; Gel filtration method | ||
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