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VACCINE PRODUCTION AGAINST Trichophyton Verrucosum ISOLATED FROM MAN AND CATTLES | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 11, Issue 1, 2012, Pages 1-13 | ||
| Authors | ||
| Shyimaa N. Yassin; Mohammed K. Faraj | ||
| Abstract | ||
| ABSTRUCT This study consist of two parts, the first part includes isolation and diagnosis of dermatophytes from infected human and animals. Ninety two samples were collected from young and adult males and females patients The percentage of fungal infections from human dermatological cases was 59.78%. Skin scraping and infected hair samples were obtained from 106 infected animals (cattles and calves) with varying ages and sex, It appeared that 44.33% of cases were infected with ringworm. After exact diagnosis of these samples, T. verrucosum was one of dematophytes that could be isolated from human and animal, and since it represent the major cause of ringworm in cattle with increased proportion of transmission to human, the second stage of this study was aimed by production of vaccine against dermatophytes to decrease the number of cases in animals and then in human. The vaccine was prepared by production of soluble antigens from sonication of veruilent strain of fungus and then inoculated in guinea pigs by using special vaccination program, and after administration of challenge dose they showed resistance at 100%. The whole cell extract of T. verrucosum was used in used in separation process by Gel Chromatography technique with using Sephadex G 100 for separation of it’s protein according to molecular weight, so 2 peaks were observed which used in induction of cell mediated immunity in sensitized animals and for identification of humeral immunity by using passive heamagghitination test to evaluate of efficacy. So the 2’nd peak was found to be possess Biological properties and antigenic ability to induce cellular immune response and serological test, to demonstrate humeral immune. | ||
| Keywords | ||
| Trichophyton verrucosum; Vaccine | ||
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